B-hPD-1/hBTLA/hHVEM mice
| Strain Name |
C57BL/6-Pdcd1tm1(PDCD)BcgenBtlatm1(BTLA)Bcgen Tnfrsf14tm1(TNFRSF14)Bcgen/Bcgen
|
Common Name | B-hPD-1/hBTLA/hHVEM mice |
| Background | C57BL/6 | Catalog number | 113166 |
|
Aliases |
AIMTBS, CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1; BTLA1, CD272; ATAR, CD270, HVEA, HVEM, LIGHTR, TR2 |
||
|
NCBI Gene ID |
5133,151888,8764 | ||
模型验证
Targeting strategy
Frequency of leukocyte subpopulations in spleen
Frequency of leukocyte subpopulations in blood
Frequency of leukocyte subpopulations in lymph nodes
Protein expression analysis in spleen
Gene targeting strategy for B-hPD -1/hBTLA/hHVEM mice. The exon 2 of mouse PD-1 gene that encodes the IgV domain was replaced by human PD-1 exon 2 in B-hPD-1 mice. The exon 2 of mouse Btla gene that encodes the extracellular domain was replaced by human BTLA exon 2 in B-hBTLA/hHVEM mice. The exons 1~6 of mouse Tnfrsf14 gene that encode the extracellular domain were replaced by human TNFRSF14 exons 1~6 in B-hBTLA/hHVEM mice.
B-hPD-1/hBTLA/hHVEM mice were obtained by mating B-hPD-1/hBTLA mice and B-hBTLA/hHVEM mice.
Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice (female, n=3, 7-week-old) and homozygous B-hPD-1/hBTLA/hHVEM mice (female, n=3, 7-week-old). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations.
Frequency of leukocyte subpopulations in blood by flow cytometry. Blood were isolated from wild-type C57BL/6 mice (female, n=3, 7-week-old) and homozygous B-hPD-1/hBTLA/hHVEM mice (female, n=3, 7-week-old). A. Flow cytometry analysis of the blood cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations.
Frequency of leukocyte subpopulations in blood by flow cytometry. Lymph nodes cells were isolated from wild-type C57BL/6 mice (female, n=3, 7-week-old) and homozygous B-hPD-1/hBTLA/hHVEM mice (female, n=3, 7-week-old). A. Flow cytometry analysis of the lymph nodes cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations.
Strain specific BTLA expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hPD-1/hBTLA/hHVEM mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hBTLA/hHVEM mice (H/H;H/H;H/H). Protein expression was analyzed with anti-mouse BTLA antibody (Biolegend, 134809) and anti-human BTLA antibody (Biolegend, 344509) by flow cytometry. Mouse BTLA was only detectable in wild-type C57BL/6 mice. Human BTLA was exclusively detectable in homozygous B-hPD-1/hBTLA/hHVEM mice, but not in wild-type C57BL/6 mice.
Strain specific HVEM expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hPD-1/hBTLA/hHVEM mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hBTLA/hHVEM mice (H/H;H/H;H/H). Protein expression was analyzed with anti-mouse HVEM antibody (Biolegend, 136305) and anti-human HVEM antibody (Biolegend, 318806) by flow cytometry. Mouse HVEM was only detectable in wild-type C57BL/6 mice. Human HVEM was exclusively detectable in homozygous B-hPD-1/hBTLA/hHVEM mice, but not in wild-type C57BL/6 mice.
Strain specific HVEM expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hPD-1/hBTLA/hHVEM mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hBTLA/hHVEM mice (H/H;H/H;H/H) stimulated without or with anti-mouse CD3ε antibody (7.5 μg, i.p.) in vivo for 24 hrs. Protein expression was analyzed with anti-mouse PD-1 antibody (Biolegend, 109104) and anti-human PD-1 antibody (Biolegend, 329904) by flow cytometry. Mouse PD-1 was only detectable in wild-type C57BL/6 mice. Human PD-1 was exclusively detectable in homozygous B-hPD-1/hBTLA/hHVEM mice, but not in wild-type C57BL/6 mice.
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