Strain specific NOTCH1 expression analysis in homozygous B-hNOTCH1 mice by flow cytometry. Thymus were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNOTCH1 mice (H/H), and analyzed by flow cytometry with species-specific anti-NOTCH1 antibody. Mouse NOTCH1 was detectable in wild-type mice. Human NOTCH1 was exclusively detectable in homozygous B-hNOTCH1 mice.
Protein expression analysis in lung
Strain specific NOTCH1 expression analysis in homozygous B-hNOTCH1 mice by flow cytometry. Lung were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNOTCH1 mice (H/H), and analyzed by flow cytometry with species-specific anti-NOTCH1 antibody. Mouse NOTCH1 was not detectable in wild-type mice. Human NOTCH1 was not detectable in homozygous B-hNOTCH1 mice.
Protein expression analysis in kidney
Strain specific NOTCH1 expression analysis in homozygous B-hNOTCH1 mice by flow cytometry. Kidney were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNOTCH1 mice (H/H), and analyzed by flow cytometry with species-specific anti-NOTCH1 antibody. Mouse NOTCH1 was not detectable in wild-type mice. Human NOTCH1 was not detectable in homozygous B-hNOTCH1 mice.