B-hMERTK mice
| Strain Name |
C57BL/6-Mertktm2(MERTK)Bcgen/Bcgen
|
Common Name | B-hMERTK mice |
| Background | C57BL/6N | Catalog number | 111278 |
|
Related Genes |
Mertk (Me; Ny; Eyk; Mer; Nyk; nmf1; nmf12) | ||
|
NCBI Gene ID |
17289 | ||
模型验证
mRNA expression analysis
Analysis of MERTK gene expression in wild-type mice and B-hMERTK mice by q-PCR. The mRNA expression of MERTK in B-hMERTK (H/H) was similar to those in the wild-type mice (+/+), demonstrating that introduction of hMERTK in place of its mouse counterpart does not change the expression level of MERTK mRNA.
Protein expression analysis
Strain specific MERTK expression analysis in homozygous B-hMERTK mice by flow cytometry. Peritoneal washes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hMERTK mice (H/H). Flow cytometry analysis of the macrophage was analyzed with species-specific anti-MERTK antibody. Mouse MERTK was detectable in wild-type mice. Human MERTK was only detectable in homozygote B-hMERTK mice but not in wild-type mice.
Analysis of leukocytes cell subpopulation in spleen
Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from male C57BL/6 and B-hMERTK mice(n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hMERTK mice were similar to those in the C57BL/6 mice, demonstrating that B-hMERTK humanized does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.
Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from male C57BL/6 and B-hMERTK mice(n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hMERTK mice were similar to those in the C57BL/6 mice, demonstrating that introduction of B-hMERTK in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in spleen. Values are expressed as mean ± SEM.
Analysis of lymph node leukocyte subpopulations by FACS.Leukocytes were isolated from male C57BL/6 and B-hMERTK mice(n=3, 7-week-old) Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis Percent of T cells, B cells, NK cells, in homozygous B-hMERTK mice were similar to those in the C57BL/6 mice, demonstrating that introduction of B-hMERTK in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.
Analysis of T cell subpopulation lymph node
Analysis of lymph node leukocyte subpopulations by FACS. Leukocytes were isolated from male C57BL/6 and B-hMERTK mice(n=3, 7- week-old) Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. Representative FACS plots. Single live CD45+ cells were gated for TCRb+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells in homozygous B-hMERTK mice were similar to those in the C57BL/6 mice, demonstrating that introduction of B-hMERTK in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.
Analysis of leukocytes cell subpopulation in blood
Analysis of blood leukocyte subpopulations by FACS. Leukocytes were isolated from male C57BL/6 and B-hMERTK mice(n=3, 7-week-old) Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hMERTK mice were similar to those in the C57BL/6 mice, demonstrating that introduction of B-hMERTKin place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.
Analysis of blood leukocyte subpopulations by FACS. Leukocytes were isolated from male C57BL/6 and B-hMERTKmice(n=3, 7-week-old) Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. Representative FACS plots. Single live CD45+ cells were gated for TCRb+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD4+, CD8+, Tregs in homozygous B-hMERTK mice were similar to those in the C57BL/6 mice, demonstrating that introduction of B-hMERTKin place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.
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