B-hIL1RAcP mice
| Strain Name | C57BL/6-Il1raptm1(IL1RAP)Bcgen/Bcgen | Common Name | B-hIL1RAcP mice |
| Background | C57BL/6 | Catalog number | 110072 |
|
Related Genes |
IL1RAP; IL1RAcP; IL1R3; C3orf13 | ||
|
NCBI Gene ID |
16180 | ||
模型验证
mRNA expression analysis
Strain specific analysis of IL1RAcP gene expression in wild type and B-hIL1RAcP mice by RT-PCR. Mouse IL1RAcP mRNA was detectable in liver of wild type mice (+/+). Human IL1RAcP mRNA was detectable only in B-hIL1RAcP mice (H/H) but not in wild type mice.
Protein expression analysis
Strain specific IL1RAcP expression analysis in homozygous B-hIL1RAcP mice by flow cytometry. Macrophages and monocytes were collected from wild type and homozygous B-hIL1RAcP mice (H/H) and analyzed by flow cytometry with species-specific anti-IL1RAcP antibody. Human IL1RAcP were exclusively detectable in homozygous B-hIL1RAcP but not wild type mice.
Functional verification of B-hIL1RAcP mice
Splenocytes were collected from wild mice and homozygous B-hIL1RAcP mice and stimulated with IL1/IL33/IL36 protein. The expression of cytokines such as IL6, TNFa, KC/GRO, IL4, IFNg, IL5, and IL12p70 in splenocyte culture medium was detected after 48 hours. The results showed that murine IL1/IL33/IL36 protein effectively activated splenocytes in wild mice and triggered the secretion of related cytokines. Murine IL1B and murine IL33 proteins could effectively activate splenocytes in B-hIL1RAcP mice and trigger the secretion of related cytokines, while murine IL36 protein could not effectively activate B-hIL1RAcP mice splenocytes.
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