B-hCLEC10A mice
| Strain Name |
C57BL/6N-Clec10atm1(CLEC10A)Bcgen/Bcgen
|
Common Name | B-hCLEC10A mice |
| Background | C57BL/6N | Catalog number | 112543 |
| Aliases | CLEC10A: HML; MGL; HML2; CD301; CLECSF13; CLECSF14 | ||
模型验证
Protein expression analysis
mRNA expression analysis
Strain specific CLEC10A expression analysis in homozygous B-hCLEC10A mice by flow cytometry. BMDC were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCLEC10A mice (H/H), and analyzed by flow cytometry with species-specific anti-CLEC10A antibody. Mouse Mgl1 and Mgl2 was detectable in wild-type mice. Human CLEC10A was exclusively detectable in homozygous B-hCLEC10A mice but not in wild-type mice.
Strain specific analysis of CLEC10A mRNA expression in wild-type C57BL/6 mice and B-hCLEC10A mice by RT-PCR. Spleen RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hCLEC10A mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CLEC10A primers. Mouse Mgl1 and Mgl2 mRNA were detectable only in wild-type C57BL/6 mice. Human CLEC10A mRNA was detectable only in homozygous B-hCLEC10A mice but not in wild-type mice.
010-56967680
info@bbctg.com.cn
Copyright © 2024 百奥赛图江苏基因生物技术有限公司. All Rights Reserved
备案号： 苏ICP备2021053911号-1
苏公网安备：32068402320845号
网站建设：北京分形科技
苏公网安备：32068402320845号
网站建设：北京分形科技