B-hCD3E/h4-1BB mice
模型验证
Protein expression analysis
Strain specific CD3E expression analysis in homozygous B-hCD3E/h4-1BB mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/h4-1BB mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3E/h4-1BB mice (H/H;H/H) but not in WT mice (+/+) .
Strain specific 4-1BB expression analysis in homozygous B-hCD3E/h4-1BB mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/h4-1BB mice (H/H;H/H) stimulated with anti-CD3ε antibody in vivo, and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse 4-1BB was detectable in WT mice (+/+). Human 4-1BB was exclusively detectable in homozygous B-hCD3E/h4-1BB mice (H/H;H/H) but not in WT mice (+/+) .
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