B-hCD30 mice
| Strain Name | C57BL/6-Cd30tm1(CD30)Bcgen/Bcgen | Common Name | B-hCD30 mice |
| Background | C57BL/6 | Catalog number |
110888 |
|
Aliases |
TNFRSF8, Ki-1, D1S166E | ||
模型验证
Protein expression analysis in T cells
Protein expression analysis in NK cells
mRNA expression analysis
Strain specific CD30 expression analysis in homozygous B-hCD30 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD30 mice (H/H) stimulated with PMA/Ionomycin in vitro, and analyzed by flow cytometry with anti-CD30 antibody. Anti-mouse CD30 antibody was cross-reactive between mouse and human. Combined with mRNA expression in wild-type and homozygous B-hCD30 mice, it can be proved that mouse CD30 was detectable in wild-type mice. HCD30 was exclusively detectable in the homozygous mice.
Strain specific CD30 expression analysis in homozygous B-hCD30 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD30 mice (H/H) stimulated with PMA/Ionomycin in vitro, and analyzed by flow cytometry with anti-CD30 antibody. Anti-mouse CD30 antibody was cross-reactive between mouse and human. Combined with mRNA expression in wild-type and homozygous B-hCD30 mice, it can be proved that mouse CD30 was detectable in wild-type mice. HCD30 was exclusively detectable in the homozygous mice.
Strain specific analysis of CD30 mRNA expression in wild-type C57BL/6 mice and B-hCD30 mice by RT-PCR. Mouse Cd30 mRNA was detectable only in splenocytes of wild-type C57BL/6 mice. Human CD30 mRNA was detectable only in homozygous B-hCD30 mice but not in wild-type mice.
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