B-hCD180 mice
| Strain Name |
C57BL/6N-Cd180tm2(CD180)Bcgen/Bcgen |
Common Name | B-hCD180 mice |
| Background | C57BL/6 | Catalog number |
111221 |
|
Related Genes |
LY64, Ly78, RP105 | ||
模型验证
Protein expression analysis in T cells
Strain specific CD180 expression analysis in homozygous B-hCD180 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD180 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD180 antibody. CD180 was not detectable in T cells.
Strain specific CD180 expression analysis in homozygous B-hCD180 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD180 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD180 antibody. Mouse CD180 was only detectable in B cells in wild-type C57BL/6 mice, while hCD180 was exclusively detectable in B cells in homozygous mice.
Strain specific CD180 expression analysis in homozygous B-hCD180 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD180 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD180 antibody. CD180 was not detectable in NK cells.
Strain specific CD180 expression analysis in homozygous B-hCD180 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD180 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD180 antibody. Mouse CD180 was only detectable in DCs in wild-type C57BL/6 mice, while hCD180 was exclusively detectable in DCs in homozygous mice.
Strain specific CD180 expression analysis in homozygous B-hCD180 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD180 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD180 antibody. CD180 was not detectable in neutrophils.
Strain specific CD180 expression analysis in homozygous B-hCD180 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD180 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD180 antibody. Mouse CD180 was only detectable in monocytes in wild-type C57BL/6 mice, while hCD180 was exclusively detectable in monocytes in homozygous mice.
Strain specific CD180 expression analysis in homozygous B-hCD180 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD180 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD180 antibody. Mouse CD180 was only detectable in macrophages in wild-type C57BL/6 mice, while hCD180 was exclusively detectable in macrophages in homozygous mice.
Strain specific CD180 expression analysis in homozygous B-hCD180 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD180 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD180 antibody. CD180 was not detectable in T cells, NK cells and neutrophils. Mouse CD180 was only detectable in B cells, DCs, monocytes and macrophages in wild-type C57BL/6 mice, while hCD180 was exclusively detectable in B cells, DCs, monocytes and macrophages in homozygous mice.
CD86 expression analysis in homozygous B-hCD180 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) (n=2, female) and homozygous B-hCD180 mice (H/H) (n=2, 1 female and 1 male), and cultured in 96-well plate with or without anti-human CD180 antibody stimulation (1, 5 μg/mL) for 24 h. Then cells were harvested and analyzed by flow cytometry. After anti-human CD180 antibody stimulation, CD86 expression was up-regulated in B cells from homozygous B-hCD180 mice (A) but not in B cells from wild-type mice (B). (C) Frequency of CD86 expression level in both wild-type and homozygous B-hCD180 mice.
MHCII expression analysis in homozygous B-hCD180 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) (n=2, female) and homozygous B-hCD180 mice (H/H) (n=2, 1 female and 1 male), and cultured in 96-well plate with or without anti-human CD180 antibody stimulation (1, 5 μg/mL) for 24 h. Then cells were harvested and analyzed by flow cytometry. After anti-human CD180 antibody stimulation, MHCII expression was up-regulated in B cells from homozygous B-hCD180 mice (A) but not in B cells from wild-type mice (B). (C) Frequency of CD86 expression level in both wild-type and homozygous B-hCD180 mice.
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