B-hCD16B mice
| Strain Name | C57BL/6-Igs2tm1(FCGR3B)Bcgen/Bcgen | Common Name | B-hCD16B mice |
| Background | C57BL/6 | Catalog number | 111863 |
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Aliases |
CD16, CD16b, FCRIIIb | ||
模型验证
Protein expression analysis in blood and spleen granulocytes
Analysis of leukocytes cell subpopulation in spleen
Analysis of T cell subpopulation in spleen
Analysis of leukocytes cell subpopulation in blood
Analysis of T cell subpopulation in blood
Analysis of leukocytes cell subpopulation in lymph nodes
Analysis of T cell subpopulation in lymph nodes
Strain specific CD16B expression analysis in homozygous B-hCD16B mice by flow cytometry. Blood (A) and spleen (B) were collected from wild-type mice (+/+) and homozygous B-hCD16B mice (H/H), and analyzed by flow cytometry with species-specific anti-CD16 antibody, respectively. Human CD16B was exclusively detectable in granulocytes of homozygous B-hCD16B mice. Note: the clone of anti-human CD16B antibody is 3G8.
Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and homozygous B-hCD16B mice (n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hCD16B mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCD16B does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.
Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and homozygous B-hCD16B mice (n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hCD16B mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCD16B does not change the overall development, differentiation or distribution of these T cell subtypes in spleen. Values are expressed as mean ± SEM.
Analysis of blood leukocyte subpopulations by FACS. Blood cells were isolated from female C57BL/6 and homozygous B-hCD16B mice (n=3, 7-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hCD16B mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCD16B does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.
Analysis of blood T cell subpopulations by FACS. Blood cells were isolated from female C57BL/6 and homozygous B-hCD16B mice (n=3, 7-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hCD16B mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCD16B does not change the overall development, differentiation or distribution of these T cell subtypes in blood. Values are expressed as mean ± SEM.
Analysis of lymph nodes leukocyte subpopulations by FACS. Lymph nodes were isolated from female C57BL/6 and homozygous B-hCD16B mice (n=3, 7-week-old). Flow cytometry analysis of the lymph nodes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hCD16B mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCD16B does not change the overall development, differentiation or distribution of these cell types in lymph nodes. Values are expressed as mean ± SEM.
Analysis of lymph nodes T cell subpopulations by FACS. Lymph nodes were isolated from female C57BL/6 and homozygous B-hCD16B mice (n=3, 7-week-old). Flow cytometry analysis of the lymph nodes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hCD16B mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCD16B does not change the overall development, differentiation or distribution of these T cell subtypes in lymph nodes. Values are expressed as mean ± SEM.
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