Strain specific analysis of CD160 gene expression in wild-type mice and B-hCD160 mice by RT-PCR. Mouse Cd160 mRNA was detectable in splenocytes of wild-type mice (+/+). Human CD160 mRNA was detectable only in homozygous B-hCD160 mice (H/H) but not in wild-type mice.
Protein expression analysis
Strain specific CD160 expression analysis in homozygous B-hCD160 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD160 mice (H/H) which sitimulated with the serial dilutions of mouse IL-2 for 18h, and analyzed by flow cytometry with species-specific anti-CD160 antibody. Mouse CD160 was detectable in wild-type mice. Human CD160 was exclusively detectable in homozygous B-hCD160 mice but not in wild-type mice.