B-hLGALS3 mice
| Strain Name |
C57BL/6N-Lgals3tm1(LGALS3)Bcgen/Bcgen |
Common Name | B-hLGALS3 mice |
| Background | C57BL/6N | Catalog number | 113086 |
|
Aliases |
GBP, L-34, Mac-2, gal3 | ||
|
NCBI Gene ID |
16854 | ||
模型验证
Protein expression analysis in plasma
mRNA expression analysis
Strain specific LGALS3 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hLGALS3 mice by ELISA. Plasma was collected from wild-type C57BL/6 mice (+/+) (n=3, 7-week-old) and homozygous B-hLGALS3 mice (H/H) (n=3, 7-week-old). Protein expression level of LGALS3 was analyzed by ELISA. Mouse LGALS3 was detectable in wild-type mice and homozygous B-hLGALS3 mice, as the kit is cross-recognize both human and mouse LGALS3. Human LGALS3 was exclusively detectable in homozygous B-hLGALS3 mice.
Strain specific analysis of LGALS3 mRNA expression in wild-type C57BL/6 mice and B-hLGALS3 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hLGALS3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human LGALS3 primers. Mouse Lgals3 mRNA was detectable only in wild-type C57BL/6 mice. Human LGALS3 mRNA was detectable only in homozygous B-hLGALS3 mice but not in wild-type mice.
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