mRNA expression analysis
Strain specific analysis of IL23A and IL12B gene expression in B-hIL23A/hIL12B mice by RT-PCR. Mouse Il23a and Il12b mRNA were detectable in thymocyte of wild-type mice (+/+) . Human IL23A and IL12B mRNA were detectable only in homozygous B-hIL23A/hIL12B mice (H/H) but not in wild-type C57BL/6 mice.
Analysis of spleen leukocyte subpopulations by FACS
Effects of anti-human IL23A antibody in psoriasis-like skin lesions model of B-hIL23A/hIL12B mice. (A) Body weight changes during treatment. (B-C) Erythema and the scaling score of the back were scored daily on a scale from 0 to 4. Additionally, the cumulative score (erythema plus scaling) is depicted (D). Results indicated that structural features of IMQ-induced skin inflammation is visible and increased in severity up to day 5. Anti-human IL23A antibody can efficiently decrease the development of the psoriasis-like disease in B-hIL23A/hIL12B mice.