B-hIL17B mice
| Strain Name | C57BL/6-Il17btm1(IL17B)Bcgen/Bcgen | Common Name | B-hIL17B mice |
| Background | C57BL/6 | Catalog number |
110089 |
| Aliases | IL17B: IL-17B, IL-20, NIRF, ZCYTO7 | ||
模型验证
Protein expression analysis
mRNA expression analysis
Western blot analysis of IL17B protein expression in homozygous B-hIL17B mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIL17B mice (H/H), and then analyzed by western blot with anti-IL17B antibody. 30 μg total proteins were loaded for western blotting analysis. IL17B was detected in liver and trachea in both wild-type and homozygous mice, as the antibody was cross-reactive between human and mouse.
Strain specific analysis of IL17B mRNA expression in wild-type C57BL/6 mice and B-hIL17B mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hIL17B mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human IL17B primers. Mouse Il17b mRNA was detectable only in wild-type C57BL/6 mice. Human IL17B mRNA was detectable only in homozygous B-hIL17B mice but not in wild-type mice.
010-56967680
info@bbctg.com.cn
Copyright © 2024 百奥赛图江苏基因生物技术有限公司. All Rights Reserved
备案号： 苏ICP备2021053911号-1
苏公网安备：32068402320845号
网站建设：北京分形科技
苏公网安备：32068402320845号
网站建设：北京分形科技