Strain specific analysis of GRP gene expression in wild-type mice and B-hGRP mice by RT-PCR. Mouse Grp mRNA was detectable in brain of wild-type mice (+/+). Human GRP mRNA was detectable only in homozygous B-hGRP but not in wild-type mice.
Strain specific GRP expression analysis in homozygous B-hGRP mice by western blot. Brain tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hGRP mice (H/H), and analyzed by western blot with anti-GRP antibody. GRP was detectable in wild type mice and homozygous B-hGRP mice, as the antibody is crossly reactive with GRP in human and mice.