Strain specific F3 expression analysis in homozygous B-hF3 mice by flow cytometry. Endotheliocytes from lung were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hF3 mice (H/H), and analyzed by flow cytometry with species-specific anti-F3 antibody. Mouse F3 was detectable in wild-type mice. Human F3 was exclusively detectable in homozygous B-hF3 mice but not in wild-type mice.
Protein expression analysis in brain
Strain specific F3 expression analysis in homozygous B-hF3 mice by flow cytometry. Endotheliocytes from brain were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hF3 mice (H/H), and analyzed by flow cytometry with species-specific anti-F3 antibody. Mouse F3 was detectable in wild-type mice. Human F3 was exclusively detectable in homozygous B-hF3 mice but not in wild-type mice.