B-hCCL8 mice
| Strain Name |
C57BL/6N-Ccl12tm1(CCL8)Bcgen/Bcgen |
Common Name | B-hCCL8 mice |
| Background | C57BL/6N | Catalog number | 111400 |
|
Aliases |
HC14, MCP2, MCP-2, SCYA8, SCYA10 | ||
|
NCBI Gene ID |
6355 | ||
模型验证
Description
- CCL8 (C-C motif chemokine ligand 8), also known as MCP-2, is a chemotactic cytokine, which was mainly produced in monocytes/macrophages, fibroblasts, endometrial cells and mast cells. CCL8 can exert a chemotactic effect on monocytes, T lymphocytes, NK cells, basophils and eosinophils.
- Biocytogen developed a CCL8 humanized mice, the exons 1~3 of mouse Ccl12 gene that encode the full length protein were replaced by human CCL8 exons 1~3 in B-hCCL8 mice. Then, confirmed that human CCL8 protein was exclusively detectable in serum of homozygous B-hCCL8 mice.
- The mice can be used for preclinical pharmacodynamics studies of target-related disease.
Protein expression analysis
Strain specific CCL8 and CCL12 expression analysis in wild-type C57BL/6N mice and homozygous humanized B-hCCL8 mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hCCL8 mice (H/H) stimulated with 40 μg LPS in vivo for 2 hours (male, 25-week old, n=3). Expression level of hCCL8 and mCCL12 were analyzed by ELISA (anti-mouse CCL12 ELISA kit: Abcam, ab246547; anti-human CCL8 ELISA kit: Abcam, ab223856). Mouse CCL12 was only detectable in wild-type C57BL/6N mice. Human CCL8 was exclusively detectable in homozygous B-hCCL8 mice but not in wild-type mice. Values are expressed as mean ± SEM.
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