| Strain Name |
C57BL/6N-Hctm1(C5)Bcgen/Bcgen |
Common Name | B-hC5 mice |
| Background | C57BL/6N | Catalog number | 110958 |
|
Aliases |
C5Da, C5b, CPAMD4, ECLZB, C5 | ||
|
NCBI Gene ID |
727 | ||
- This C5 gene encodes a component of the complement system. It's part of the innate immune system and important for inflammation, host homeostasis and defense against pathogens. The encoded preproprotein generates multiple products like the C5 alpha chain, beta chain, C5a anaphylatoxin, and C5b. C5 protein consists of alpha and beta chains linked by a disulfide bridge. Cleavage of the alpha chain forms C5a anaphylatoxin with spasmogenic and chemotactic activity and C5b. Mutations cause complement component 5 deficiency with recurrent bacterial infections.
- Gene targeting strategy for B-hC5 mice: The CDS of the human C5 gene (excluding exon 1 and part of exon 2) and the mouse 3’UTR were inserted into the exon 2 of the mouse Hc gene in B-hC5 mice. The promoter, 5’UTR, and 3’UTR region of the mouse gene were also retained. The chimeric human C5 expression was driven by endogenous mouse Hc promoter, while mouse Hc gene transcription and translation will be disrupted.
- B-hC5 mice cannot initiate the classical complement pathway, so B-hC5 mice cannot be used for disease model establishment.
Targeting strategy
Protein expression analysis in serum
Strain-specific C5 expression analysis in wild-type C57BL/6 mice and homozygous B-hC5 mice by ELISA. Serum was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hC5 mice (H/H) (female, 8 weeks old, n=3 and male, 8 weeks old, n=3). Expression levels of mouse and human C5 were analyzed by ELISA (anti-mouse C5 ELISA kit: abcam, ab264609; anti-human C5 ELISA kit: abcam, ab125963). Mouse C5 was only detectable in wild-type C57BL/6 mice. Human C5 was exclusively detectable in homozygous B-hC5 mice. Values are expressed as mean ± SEM.
Strain-specific C5a expression analysis in wild-type C57BL/6 mice and homozygous B-hC5 mice by ELISA. Serum was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hC5 mice (H/H) ) (female, 8 weeks old, n=3 and male, 8 weeks old, n=3). Expression levels of mouse and human C5a were analyzed by ELISA (anti-mouse C5a ELISA kit: abcam, ab193718; anti-human C5a ELISA kit: abcam, ab193695). Mouse C5a was only detectable in wild-type C57BL/6 mice. Human C5a was exclusively detectable in homozygous B-hC5 mice. Values are expressed as mean ± SEM.
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