B-hGPR55 mice
| Strain Name |
C57BL/6N-Gpr55tm1(GPR55)Bcgen/Bcgen
|
Common Name | B-hGPR55 mice |
| Background | C57BL/6N | Catalog number | 112462 |
|
Aliases |
LPIR1 | ||
模型验证
mRNA expression analysis
Strain specific analysis of GPR55 mRNA expression in wild-type C57BL/6 mice and B-hGPR55 mice by RT-PCR. Brain RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hGPR55 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GPR55 primers. Mouse Gpr55 mRNA was detectable only in wild-type C57BL/6 mice. Human GPR55 mRNA was detectable only in homozygous B-hGPR55 mice but not in wild-type mice.
Western blot analysis of GPR55 protein expression in homozygous B-hGPR55 mice. Various tissue lysates were collected from C57BL/6N wildtype mice (+/+) and homozygous B-hGPR55 mice (H/H), and then analyzed by western blot with anti-GPR55 antibody. 40 μg total proteins were loaded for western blotting analysis. GPR55 was detected in brain, pancreas, liver and stomach in homozygous B-hGPR55 mice. （The arrow above indicates the glycosylated GPR55 protein, the arrows below indicates the GPR55 protein）
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