B-hCXCR4 mice
| Strain Name |
C57BL/6-Cxcr4tm1(CXCR4)Bcgen/Bcgen
|
Common Name | B-hCXCR4 mice |
| Background | C57BL/6 | Catalog number | 110100 |
|
Related Genes |
CXC-R4, CXCR-4, CD184 | ||
|
NCBI Gene ID |
12767 | ||
模型验证
mRNA expression analysis
Strain specific analysis of CXCR4 gene expression in WT and B-hCXCR4 mice by RT-PCR. Mouse Cxcr4 mRNA was detectable in splenocytes and thymocytes of wild-type (+/+). Human CXCR4 mRNA was detectable only in H/H, but not in +/+ mice.
Strain specific CXCR4 expression analysis in homozygous B-hCXCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCXCR4 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific CXCR4 antibody. Mouse CXCR4 was detectable in WT mice. Human CXCR4 was exclusively detectable in homozygous B-hCXCR4, but the expression level was significantly lower than mouse CXCR4 in the C57BL/6 mice.
Protein expression analysis in CD4+ T cells
Strain specific CXCR4 expression analysis in homozygous B-hCXCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCXCR4 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific CXCR4 antibody. Mouse CXCR4 was detectable in WT mice. Human CXCR4 was exclusively detectable in homozygous B-hCXCR4, but the expression level was significantly lower than mouse CXCR4 in the C57BL/6 mice.
Strain specific CXCR4 expression analysis in homozygous B-hCXCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCXCR4 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific CXCR4 antibody. Mouse CXCR4 was detectable in WT mice. Human CXCR4 was exclusively detectable in homozygous B-hCXCR4, but the expression level was significantly lower than mouse CXCR4 in the C57BL/6 mice.
010-56967680
info@bbctg.com.cn
Copyright © 2024 百奥赛图江苏基因生物技术有限公司. All Rights Reserved
备案号： 苏ICP备2021053911号-1
苏公网安备：32068402320845号
网站建设：北京分形科技
苏公网安备：32068402320845号
网站建设：北京分形科技