B-Uox KO mice plus
| Strain Name |
C57BL/6JNifdc-Uoxtm2Bcgen/Bcgen
|
Common Name | B-Uox KO mice plus |
| Background | C57BL/6JNifdc | Catalog number | 113135 |
|
Aliases |
NA | ||
模型验证
Description
In vivo efficacy of Allopurinol
- Uox mainly expressed in the liver and involved in allantoin metabolic process, purine-containing compound catabolic process and urate catabolic process.
- The exons 3~4 of mouse Uox gene were knocked out in B-Uox KO mice plus (C57BL/6J).
- It was proved that there was no expression of Uox protein in the liver of B-Uox KO mice and the uric acid in the blood was significantly increased.
- Allopurinol could significantly reduce the serum uric acid level of B-Uox KO mice plus.
- B-Uox KO mice plus can be used for pharmacological evaluation of hyperuricemia, gout, etc.
Protein expression analysis in B-Uox mice plus
Western blot analysis of Uox protein expression in homozygous B-Uox KO mice plus. Liver, kidney, lung, spleen, heart, and brain were collected from C57BL/6 wild-type mice (+/+) and homozygous B-Uox KO mice plus (-/-)， and then analyzed by western blot with species-specific anti-Uox antibody (Santa cruz，sc-166214). 40 μg total proteins were loaded for western blotting analysis. Uox was not detected in liver of homozygous B-Uox KO mice plus (-/-).
Efficacy validation in B-Uox KO mice plus. Mice were divided into 3 groups (male, 6 weeks), C57BL/6 (n=4), B-Uox KO mice plus (n=1), B-Uox KO mice plus with 100μg/ml allopurinol in drinking water from 6 weeks to 8 weeks (n=4). Compared with C57BL/6 mice (+/+), serum uric acid level of B-Uox KO mice plus (-/-) was significantly increased, and the intervention of allopurinol could significantly reduce the serum uric acid level of B-Uox KO mice plus (-/-). Values are expressed as mean ± SEM.
Efficacy validation in B-Uox KO mice plus. Mice were divided into 2 groups (male, 10-11 weeks-old, n=2), of B-Uox KO mice plus and of B-Uox KO mice plus with 100μg/ml allopurinol in drinking water. Allopurinol could significantly reduce the serum uric acid level of B-Uox KO mice plus (-/-). Values are expressed as mean ± SEM.
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