B-Rag2 KO mice(C)
| Strain Name | BALB/cCrSlcNifdc-Rag2tm1Bcgen/Bcgen | Common Name | B-Rag2 KO mice(C) |
| Background | BALB/cCrSlcNifdc | Catalog number | 112883 |
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Aliases |
RAG-2 |
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NCBI Gene ID |
19374 | ||
模型验证
The size and weight of thymus and spleen significantly reduced in B-Rag2 KO mice(C)
Frequency of leukocyte subpopulations in blood
Frequency of leukocyte subpopulations in bone marrow
The size and weight of thymus and spleen of B-Rag2 KO mice(C) were significantly reduced compared to that of wild-type BALB/c mice. Thymuses, spleens and lymph nodes were isolated from wild-type BALB/c mice and B-Rag2 KO mice(C) (n=3, 7-week-old). A. Gross anatomy of thymuses; B. The size of spleen, thymus and lymph node; C. Comparison of the weight of spleen and thymus in wild-type BALB/c mice and B-Rag2 KO mice(C). Results showed that the size and weight of spleens and thymuses in B-Rag2 KO mice(C) were significantly reduced compared to that in wild-type BALB/c mice. Lymph nodes were not visualized in B-Rag2 KO mice(C).
Frequency of leukocyte subpopulations in spleen
Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from female wild-type BALB/c mice (n=3, 7-week-old) and homozygous B-Rag2 KO mice(C) (n=3, 7-week-old). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. The frequency of total T cells, CD4+ T cells, CD8+ T cells, Tregs and B cells were decreased in B-Rag2 KO mice(C). The frequency of NK cells, DCs, neutrophils and macrophages were increased in B-Rag2 KO mice(C) compared to BALB/c mice, demonstrating that Rag2 was successfully knocked out in spleen. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.
Frequency of leukocyte subpopulations in blood by flow cytometry. Blood cells were isolated from female wild-type BALB/c mice (n=3, 7-week-old) and homozygous B-Rag2 KO mice(C) (n=3, 7-week-old). A. Flow cytometry analysis of the blood cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. The frequency of total T cells, CD4+ T cells, CD8+ T cells, Tregs and B cells were decreased in B-Rag2 KO mice(C). The frequency of NK cells, DCs, neutrophils and macrophages were increased in B-Rag2 KO mice(C) compared to BALB/c mice, demonstrating that Rag2 was successfully knocked out in blood. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.
Frequency of leukocyte subpopulations in bone marrow by flow cytometry. Bone marrow cells were isolated from female wild-type BALB/c mice (n=3, 7-week-old) and homozygous B-Rag2 KO mice(C) (n=3, 7-week-old). A. Flow cytometry analysis of the bone marrow cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. The frequency of total T cells, CD4+ T cells, CD8+ T cells, Tregs and B cells were decreased in B-Rag2 KO mice(C). The frequency of NK cells, DCs, neutrophils and macrophages were increased in B-Rag2 KO mice(C) compared to BALB/c mice, demonstrating that Rag2 was successfully knocked out in bone marrow. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.
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