B-OX2R KO mice
| Strain Name | C57BL/6JNifdc-Hcrtr2tm1Bcgen/Bcgen | Common Name | B-OX2R KO mice |
| Background | C57BL/6JNifdc | Catalog number | 113614 |
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Aliases |
ORXR2, OX2R, OXR2 |
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NCBI Gene ID |
3062 | ||
模型验证
Description
mRNA expression analysis
- Orexin, a neuropeptide synthesized by hypothalamic neurons, exists in two distinct forms: orexin A and orexin B. These two types of orexin exert their physiological effects by binding to two specific G-protein-coupled receptors, namely orexin receptor 1 and orexin receptor 2. Any abnormalities in the functions of orexin and its associated receptors can disrupt the body's normal sleep-wake regulatory mechanism.
- Gene targeting strategy for B-OX2R KO mice. The exons 1-7 of mouse Ox2r gene that encode the full-length protein were knocked out in B-OX2R KO mice.
- mRNA expression analysis: Mouse Ox2r mRNA was detectable only in wild-type C57BL/6 mice but not in homozygous B-OX2R KO mice.
- Application: This product is used for pharmacodynamics evaluation of Narcolepsy.
Targeting strategy
Gene targeting strategy for B-OX2R KO mice. The exons 1-7 of mouse Ox2r gene that encode the full-length protein were knocked out in B-OX2R KO mice.
Strain specific analysis of Ox2r mRNA expression in wild-type C57BL/6 mice and B-OX2R KO mice by RT-PCR. Brain RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-OX2R KO mice (-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Ox2r primers. Mouse Ox2r mRNA was detectable only in wild-type C57BL/6 mice but not in homozygous B-OX2R KO mice.
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