B-NDG Fah KO mice
| Strain Name |
NOD.CB17-Prkdcscid Il2rgtm1Bcgen Fahtm1Bcgen/Bcgen |
Common Name | B-NDG Fah KO mice |
| Background | B-NDG | Catalog number | 112959 |
|
Aliases |
NA |
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|
NCBI Gene ID |
2184 | ||
模型验证
Description
Gene targeting strategy for B-NAG Fah KO mice. The exons 2-14 of mouse Fah gene were knocked out in B-NDG Fah KO mice.
mRNA expression analysis in B-NDG Fah KO mice
Protein expression analysis in B-NDG Fah KO mice
- Fah is the final enzyme in the tyrosine metabolism pathway, primarily expressed in the liver and kidneys. Deficiency of FAH is associated with Type 1 hereditary tyrosinemia (HT). As a result of the severe liver damage induced by Fah knockout (KO), this mouse model allows for the reconstruction of a functional human liver utilizing primary human hepatocytes.
- Gene editing strategy: The exons 2-14 of mouse Fah gene were knocked out in B-NDG Fah KO mice.
- mRNA expression analysis: Mouse Fah mRNA were detectable only in B-NDG mice but not in homozygous B-NDG Fah KO mice.
- Protein expression analysis: Fah was only detected in liver and kidney of B-NDG mice but not in homozygous B-NDG Fah KO mice.
- Application: This product is used for human hepatocytes transplantation and for evaluating the efficacy and safety of drugs associated with liver diseases.
Targeting strategy
Strain specific analysis of Fah mRNA expression in B-NDG mice and B-NDG Fah KO mice by RT-PCR. Liver RNA was isolated from B-NDG mice (+/+) and homozygous B-NDG Fah KO mice (-/-). cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Fah primers. Mouse Fah mRNA was detectable only in B-NDG mice but not in homozygous B-NDG Fah KO mice.
Western blot analysis of Fah protein expression in homozygous B-NDG Fah KO mice. Various tissue lysates were collected from B-NDG mice (+/+) and homozygous B-NDG Fah KO mice (-/-), and then analyzed by western blot with anti-Fah antibody (proteintech, 14928-1-AP). A total of 40 μg of protein was loaded for western blotting analysis. Fah was only detected in liver and kidney of B-NDG mice.
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