B-Abcb4 KO mice(C)
| Strain Name |
BALB/cCrSlcNifdc-Abcb4tm1 Bcgen/Bcgen
|
Common Name | B-Abcb4 KO mice(C) |
| Background | BALB/cCrSlcNifdc | Catalog number | 112978 |
|
Related Genes |
GBD1, ICP3, MDR2, MDR3, PGY3, ABC21, MDR2/3, PFIC-3 | ||
|
NCBI Gene ID |
5244 | ||
模型验证
mRNA expression analysis in B-Abcb4 KO mice(C)
H&E staining of B-Abcb4 KO mice(C)
Sirius red staining of B-Abcb4 KO mice(C)
Mouse Abcb4 mRNA expression in wild-type BALB/C mice and B-Abcb4 KO mice(C) by RT-PCR. Liver and lung RNA were isolated from wild-type BALB/C mice (+/+) and B-Abcb4 KO mice(C) (-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Abcb4 primers. Mouse Abcb4 mRNA was detectable only in wild-type BALB/C mice, but not in B-Abcb4 KO mice(C) (-/-).
H&E staining of B-Abcb4 KO mice(C). Liver tissues from BALB/C mice and B-Abcb4 KO mice(C) (n=3, Male, 6 week-old; n=2, Female, 6 week-old) were collected and analyzed for H&E staining. There were no abnormal changes in the liver of wild-type BALB/C mice, but in the liver of B-Abcb4 KO mice, proliferation of intrahepatic bile ducts, thickening of the duct walls, and a small amount of inflammatory cell infiltration could be observed. (a) Bile ducts (b) Inflammatory
Sirius red staining of B-Abcb4 KO mice(C). (A) Representative images of Sirius red staining showing liver fibrosis in wild-type BALB/C mice (+/+) and B-Abcb4 KO mice(C) (-/-) (n=3, Male, 6 week-old; n=2, Female, 6 week-old). (B) Statistic data of Sirius red staining. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001.
Biochemistry analysis
Biochemical test of B-Abcb4 KO mice(C). Values are expressed as mean ± SEM. N=6 mice per group. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
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