B-hORAI1 mice
| Strain Name |
C57BL/6N-Orai1tm1(ORAI1)Bcgen/Bcgen |
Common Name | B-hORAI1 mice |
| Background | C57BL/6N | Catalog number |
111617 |
|
Aliases |
orai-1; Tmem142a; D730049H07Rik |
||
模型验证
Protein expression analysis
mRNA expression analysis
Western blot analysis of ORAI1 protein expression in homozygous B-hORAI1 mice. Various tissue lysates were collected from C57BL/6 wildtype mice (+/+) and homozygous B-hORAI1 mice (H/H) , and then analyzed by western blot with species-specific anti-ORAI1 antibody. 32 μg total proteins were loaded for western blotting analysis. Human ORAI1 was detected in spleen/lung/kidney/liver and small intestine of wildtype mice (+/+) and homozygous B-hORAI1 mice (H/H), as the antibody is partly crossly reactive with human and mice.
Strain specific analysis of ORAI1 mRNA expression in wild-type C57BL/6 mice and B-hORAI1 mice by RT-PCR. Spleen and lung RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hORAI1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ORAI1 primers. Mouse Orai1 mRNA was detectable only in wild-type C57BL/6 mice. Human ORAI1 mRNA was detectable only in homozygous B-hORAI1 mice but not in wild-type mice.
010-56967680
info@bbctg.com.cn
Copyright © 2024 百奥赛图江苏基因生物技术有限公司. All Rights Reserved
备案号： 苏ICP备2021053911号-1
苏公网安备：32068402320845号
网站建设：北京分形科技
苏公网安备：32068402320845号
网站建设：北京分形科技