Strain specific MCAM expression analysis in homozygous B-hMCAM mice by western blot.
Kidney, small intestine and testis were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hMCAM mice (H/H), and analyzed by western blot with anti-MCAM antibody. Mouse MCAM was detectable in wild-type mice. Human MCAM was exclusively detectable in homozygous B-hMCAM mice but not in wild-type mice.