B-hCSPG5 mice
| Strain Name | C57BL/6-Cspg5tm2(CSPG5)Bcgen/Bcgen | Common Name | B-hCSPG5 mice |
| Background | C57BL/6 | Catalog number |
112235 |
|
Aliases |
CSPG5: NGC |
||
模型验证
Protein expression analysis
mRNA expression analysis
Western blot analysis of CSPG5 protein expression in homozygous B-hCSPG5 mice. Cortex lysates were collected from C57BL/6 wild-type (+/+) mice and homozygous B-hCSPG5 mice (H/H), and then analyzed by western blot with species-specific anti-CSPG5 antibody. 40 μg total proteins were loaded for western blotting analysis. CSPG5 protein was detected in the cortex of wild-type C57BL/6 mice and homozygous B-hCSPG5 mice, as the antibody is cross-recognize both human and mouse CSPG5. It is impossible to conclude from the data that the observed three bands are CSPG5 three isoforms.
Strain specific analysis of CSPG5 mRNA expression in wild-type C57BL/6 mice and B-hCSPG5 mice by RT-PCR. Cortex RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCSPG5 mice(H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CSPG5 primers. Mouse Cspg5 mRNA was detectable only in wild-type C57BL/6 mice. Human CSPG5 mRNA was detectable only in homozygous B-hCSPG5 mice but not in wild-type mice.
010-56967680
info@bbctg.com.cn
Copyright © 2024 百奥赛图江苏基因生物技术有限公司. All Rights Reserved
备案号： 苏ICP备2021053911号-1
苏公网安备：32068402320845号
网站建设：北京分形科技
苏公网安备：32068402320845号
网站建设：北京分形科技