B-hPTHrP mice
| Strain Name |
C57BL/6N-Pthrptm1(PTHrP)Bcgen/Bcgen
|
Common Name | B-hNGAL mice |
| Background | C57BL/6N | Catalog number |
111017 |
| Aliases |
PTHrP: HHM; PLP; BDE2; PTHR; PTHRP |
||
模型验证
Protein expression analysis
mRNA expression analysis
Western blot analysis of PTHrP protein expression in homozygous B-hPTHrP mice. Various tissue lysates were collected from C57BL/6 wildtype (+/+) mice and homozygous B-hPTHrP (H/H) mice, and then analyzed by western blot with anti-PTHrP antibody. 40 μg total proteins were loaded for western blotting analysis. PTHrP was detected in brain, stomach, kidney and spleen.
Strain specific analysis of PTHRP mRNA expression in wild-type C57BL/6 mice and B-hPTHrP mice by RT-PCR. Bladder and brain RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hPTHrP mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human PTHrP primers. Mouse Pthrp mRNA was detectable only in wild-type C57BL/6 mice. Human PTHRP mRNA was detectable only in homozygous B-hPTHrP mice but not in wild-type mice.
010-56967680
info@bbctg.com.cn
Copyright © 2024 百奥赛图江苏基因生物技术有限公司. All Rights Reserved
备案号： 苏ICP备2021053911号-1
苏公网安备：32068402320845号
网站建设：北京分形科技
苏公网安备：32068402320845号
网站建设：北京分形科技