B-hCD40/hFcRn mice
| Strain Name |
C57BL/6-Cd40tm1(CD40)Bcgen Fcgrttm1(FCGRT)Bcgen/Bcgen
|
Common Name | B-hCD40/hFcRn mice |
| Background | C57BL/6 | Catalog number | 111912 |
|
Related Genes |
CD40 (Bp50, CDW40, TNFRSF5, p50) FCGRT (FCRN, FcgammaRn, alpha-chain) |
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模型验证
Protein expression analysis
Strain specific analysis of CD40 expression in homozygous B-hCD40/hFcRn mice by flow cytometry. Splenocytes were collected from C57BL/6 mice (+/+;+/+) and homozygous B-hCD40/hFcRn mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD40 antibody. Mouse CD40 was detectable in wild-type mice. Human CD40 was exclusively detectable in homozygous B-hCD40/hFcRn mice but not in wild-type mice.
Strain specific analysis of FcRn expression in homozygous B-hCD40/hFcRn mice by western blot.Liver was collected from C57BL/6 mice (+/+;+/+) and homozygous B-hCD40/hFcRn mice (H/H;H/H) and analyzed by western blot with species-specific anti-FcRn antibody. Mouse FcRn was detectable in wild-type mice. Human FcRn was detectable only in homozygous B-hCD40/hFcRn mice but not in wild-type mice.
Determination of plasma concentrations of human IgG in different mice
Determination of plasma concentrations of human IgG in different mice
The study evaluated the pharmacokinetic characteristics of anti-human CD40 antibodies in B-hCD40/hFcRn mice. C57BL/6 mice, B-hFcRn mice, and B-hCD40/hFcRn mice were treated with YH015 or YH003 (anti-human CD40 antibodies) (n=6). Blood samples were collected at different time points for the pharmacokinetic (PK) assay. Compared to wild-type mice and B-hFcRn mice, B-hCD40/hFcRn mice can more accurately reflect the in vivo concentration changes of anti-human CD40 antibodies, the results showed that B-hCD40/hFcRn mice can be used to evaluate the pharmacokinetics of anti-human CD40 antibodies in vivo.
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