B-hMSLN ID8|BioMice百奥动物

Common name	B-hMSLN ID8	Catalog number	321823
Aliases	MPF, SMRP	Disease	Ovarian Cancer
Organism	Mouse	Strain	C57BL/6
Tissue types	Ovary	Tissue	Ovary

模型验证

Description

The human MSLN coding sequences were inserted to mouse Msln gene exon 2 in B-hMSLN ID8 cells, so that the mouse MSLN was not expressed anymore, human MSLN was highly expressed on the surface of B-hMSLN ID8 cells.

Application

B-hMSLN ID8 cells have the capability to establish tumors in C57BL/6N mice in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hMSLN ID8 cells. The exogenous promoter and human MSLN CDS was inserted to mouse Msln gene exon 2 in B-hMSLN ID8 cells.

Protein expression analysis

MSLN expression analysis in B-hMSLN ID8 cells by flow cytometry. Single cell suspensions from B-hMSLN ID8 cultures were stained with species-specific anti-MSLN antibody. Human MSLN were detected on the surface of B-hMSLN ID8 cells. The 2-A11 clone of B-hMSLN ID8 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hMSLN ID8 cells. B-hMSLN ID8 cells (1x10⁶, 3x10⁶) and wild-type ID8 cells (1x10⁵) were subcutaneously implanted into C57BL/6 mice. Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 × long diameter × short diameter². As shown in panel A, B-hMSLN ID8 cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells

B-hMSLN ID8 cells were subcutaneously transplanted into C57BL/6 mice (n=8). At the end of the experiment, tumor cells were harvested and assessed for human MSLN expression by flow cytometry. As shown, human MSLN was highly expressed on the surface of tumor cells. Therefore, B-hMSLN ID8 cells can be used for in vivo efficacy studies of novel MSLN therapeutics.