link', 'link', 'style', 'embed', 'object', 'frame', 'layer', 'title', 'bgsound', 'base', 'onload', 'onunload', 'onchange', 'onsubmit', 'onreset', 'onselect', 'onblur', 'onfocus', 'onabort', 'onkeydown', 'onkeypress', 'onkeyup', 'onclick', 'ondblclick', 'onmousedown', 'onmousemove', 'onmouseout', 'onmouseover', 'onmouseup', 'alert', 'eval', 'DROP', 'SELECT', 'UPDATE', 'INSERT' ]; var regexStr = unsafeKeywords.join('|'); var regex = new RegExp(regexStr, 'i'); return !regex.test(str); } function containsSpecialCharsExceptDotUnderscore(str) { // 正则表达式，匹配允许的字符集（中文、英文、数字、指定标点符号） var allowedPattern = /^[\uAC00-\uD7AF\u3000-\u303f\u3040-\u309f\u30a0-\u30ff\uff00-\uff9f\u4e00-\u9fa5a-zA-Z0-9，。，.,\s"“”\-（）()]*$/u; // 使用正则表达式测试整个字符串是否只包含允许的字符 return allowedPattern.test(str); } new Guestbook(null, '', { // 绑定验证事件, 主要使用于非弹窗信息提示; 友情提示：弹窗提示时使用可能会存在小瑕疵 validate: function(gb, trigger) { }, // 绑定提交事件 trigger: function(gb, trigger) { document.getElementById('submit').onclick = trigger; }, // 验证规则, true: 返回全部错误信息, false: 返回第一条错误信息 allError: false, // 数据处理 parse: { 'name': function(gb) { var vals = ''; var eles = gb.getEleByName('name')[0]; vals = eles.value; if(vals == "姓名"){ vals = ""; } return vals; }, }, // 提交成功回调方法 onSuccess: function(success) { // {status: 1, 'info': "成功信息", field: "字段名"} my_alert('信息已提交'); $('#')[0].reset(); $('#guojia_3 .select-val input').val(''); $('#shengfen_3 .select-val').html(''); $('#chengshi_3 .select-val').html(''); setTimeout(function(){ window.location.reload(); }, 2000); }, // 提交失败回调方法 onError: function(error) { // {status: 0, 'info': "错误信息", field: "字段名"} my_alert(error.info); } }, function(valid, gb) { var guojia = $.trim($("input[id='guojia']").val()); if (guojia == ""){ my_alert('请选择国家'); return false; } var shengfen = $.trim($("input[id='shengfen']").val()); if (shengfen == ""){ my_alert('请填写省份'); return false; } if(containsSpecialCharsExceptDotUnderscore(shengfen) == false || containsUnsafeKeyword(shengfen) == false){ my_alert('省份不应包含特殊字符或禁用词'); return false; } var chengshi = $.trim($("input[id='chengshi']").val()); if (chengshi == ""){ my_alert('请填写城市'); return false; } if(containsSpecialCharsExceptDotUnderscore(chengshi) == false || containsUnsafeKeyword(chengshi) == false){ my_alert('城市不应包含特殊字符或禁用词'); return false; } var title = $.trim($("input[id='name']").val()); if (title == ""){ my_alert('请填写姓名'); return false; } if (title.length < 2 || title.length > 20){ my_alert('姓名控制在2-20个字符之内'); return false; } if(containsSpecialCharsExceptDotUnderscore(title) == false || containsUnsafeKeyword(title) == false){ my_alert('姓名不应包含特殊字符或禁用词'); return false; } //tel var phone = $.trim($("input[id='tel']").val()); var re = /^[0-9]+.?[0-9]*/; if (phone == ""){ my_alert('请填写联系电话'); return false; } if (!re.test(phone)) { my_alert("请输入纯数字"); return false; 　　} if (phone.length != 11){ my_alert('请输入正确的电话号码'); return false; } if(containsSpecialCharsExceptDotUnderscore(phone) == false || containsUnsafeKeyword(phone) == false){ my_alert('联系电话不应包含特殊字符或禁用词'); return false; } var danwei = $.trim($("input[id='danwei']").val()); if (danwei == ""){ my_alert('请填写单位'); return false; } if(containsSpecialCharsExceptDotUnderscore(danwei) == false || containsUnsafeKeyword(danwei) == false){ my_alert('单位不应包含特殊字符或禁用词'); return false; } var zhiwei = $.trim($("input[id='zhiwei']").val()); if (zhiwei == ""){ my_alert('请填写职位'); return false; } if(containsSpecialCharsExceptDotUnderscore(zhiwei) == false || containsUnsafeKeyword(zhiwei) == false){ my_alert('职位不应包含特殊字符或禁用词'); return false; } var email = $("input[id='email']").val(); var reg = /^\w+((-\w+)|(\.\w+))*\@[A-Za-z0-9]+((\.|-)[A-Za-z0-9]+)*\.[A-Za-z0-9]+$/; if (email == ""){ my_alert('请填写邮箱'); return false; } if (!reg.test(email)) { my_alert('请输入正确的邮箱'); return false; } if(containsUnsafeKeyword(email) == false){ my_alert('邮箱不应包含禁用词'); return false; } var verify = $.trim($("input[id='verify_img']").val()); if (verify == ""){ my_alert('请输入验证码'); return false; } return true; // 提交表单 });

动物&细胞模型

基因人源化鼠

GPCR人源化小鼠

B-hGPR87 mice

Database

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Strain Name	C57BL/6N-Gpr87^{tm1(GPR87)Bcgen}/Bcgen	Common Name	B-hGPR87 mice
Background	C57BL/6N	Catalog number	112529
Aliases	FKSG78, GPR95, KPG_002

模型验证

mRNA expression analysis

Strain specific analysis of GPR87 mRNA expression in wild-type C57BL/6 mice and B-hGPR87 mice by RT-PCR. Lung RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hGPR87 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GPR87 primers. Mouse Gpr87 mRNA was detectable only in wild-type C57BL/6 mice. Human GPR87 mRNA was detectable only in homozygous B-hGPR87 mice but not in wild-type mice.

Protein expression analysis

Western blot analysis of GPR87 protein expression in homozygous B-hGPR87 mice. Various tissue lysates were collected from C57BL/6N wildtype (+/+) mice and homozygous B-hGPR87 (H/H) mice, and then analyzed by western blot with anti-GPR87 antibody. 40 μg total proteins were loaded for western blotting analysis. GPR87 was detected in stomach, lung, esophagus and bladder.